Tris (hydroxymethyl) aminomethane

☑ Tris buffer sikuti imagwiritsidwa ntchito kwambiri ngati chosungunulira cha nucleic acid ndi mapuloteni, komanso imakhala ndi ntchito zambiri zofunika.Tris idagwiritsidwa ntchito pakukula kwa kristalo wa mapuloteni pansi pazikhalidwe zosiyanasiyana za pH.
☑ Mphamvu yotsika ya ionic ya Tris buffer ingagwiritsidwe ntchito kupanga ulusi wapakati wa lamin mu C. elegans.
☑ Tris ndi chimodzi mwazinthu zazikulu za protein electrophoresis buffer.
☑Kuonjezera apo, Tris ndi yapakatikati pokonza zopangira ma surfactants, ma vulcanization accelerators ndi mankhwala ena.Tris imagwiritsidwanso ntchito ngati mulingo wa titration.
☑Trimethylaminomethane imagwiritsidwa ntchito kwambiri mu acidemia ya metabolic komanso kupuma.Ndi chitetezo chamchere ndipo chimakhala ndi chitetezo chabwino pa metabolic acidosis ndi zochita za enzyme.
☑Tris imagwiritsidwa ntchito ngati biological buffer, ndipo pH yake ndi 6.8, 7.4, 8.0, 8.8.Kapangidwe kake, pH mtengo umasintha kwambiri ndi kutentha.Nthawi zambiri, pH mtengo umatsika ndi 0.03 pamene kutentha kumawonjezeka ndi digiri imodzi.Tris imagwiritsidwa ntchito kwambiri pokonzekera njira yochepetsera mu biochemistry ndi kuyesa kwa ma molekyulu a biology.Mwachitsanzo, ma buffer onse a TAE ndi tbe (omwe amagwiritsidwa ntchito pakusungunuka kwa nucleic acid) omwe amagwiritsidwa ntchito kwambiri poyesa zamankhwala am'chilengedwe amafunikira Tris.Chifukwa ili ndi magulu a amino, imatha kuchitapo kanthu ndi aldehydes.
☑ Tris ndi maziko ofooka, ndipo PKA yake ndi 8.1 kutentha (25 ℃).Malinga ndi chiphunzitso cha buffer, mtundu wogwira ntchito wa Tris buffer uli pakati pa 7.0 ndi 9.2.Mtengo wa pH wa Tris base aqueous solution ndi pafupifupi 10.5.Nthawi zambiri, hydrochloric acid imawonjezedwa kuti isinthe pH kukhala mtengo womwe mukufuna kuti mupeze yankho la bafa ndi pH iyi.Nthawi yomweyo, tiyenera kulabadira zotsatira za kutentha pa pKa ya Tris.Chifukwa Tris buffer ndi yankho lofooka la alkaline, DNA imachotsedwa munjira yotere kuti isungunuke.Anthu nthawi zambiri amawonjezera EDTA mu Tris hydrochloric acid buffer kuti apange "Te buffer", yomwe imagwiritsidwa ntchito kukhazikika kwa DNA ndikusunga.Ngati njira ya asidi yosinthira pH yalowa m'malo ndi acetic acid, "Tae buffer" (Tris / acetate / EDTA) imapezedwa, ndipo "tbe buffer" (Tris / borate / EDTA) imapezedwa ndikuyika boric acid. .Ma buffer awiriwa nthawi zambiri amagwiritsidwa ntchito poyesa ma nucleic acid electrophoresis.